Rao et al., 2014 ). The contact domain and CD19 + B cell super-enhancer (
) encompassing rs539846 are labeled in pink and yellow, respectively. (B) 4C-seq analyses in MEC1 CLL cells indicate the formation of a loop domain between rs539846 and cis -regulatory elements. The 4C viewpoint (green box) lies adjacent to rs539846 (dotted line). A 10-kb masked region (gray box) is also marked. ENCODE ChIP-seq data from GM12878 cells show the correspondence between loop formation and CTCF and RELA transcription factor occupancy. Canonical transcripts and chromosome 15 position also are shown. " width="100%" height="100%">
Journal: Cell Reports
Article Title: Genetic Predisposition to Chronic Lymphocytic Leukemia Is Mediated by a BMF Super-Enhancer Polymorphism
doi: 10.1016/j.celrep.2016.07.053
Figure Lengend Snippet: Contact Profile of the BMF 4C Viewpoint in Combination with ChIP-Seq Data (A) Heatmap representing chromatin interactions in GM12878 cells at 40.32–40.52 Mb on chromosome 15. Chromatin contact domains called by the Arrowhead algorithm are marked by white lines ( Rao et al., 2014 ). The contact domain and CD19 + B cell super-enhancer ( Hnisz et al., 2013 ) encompassing rs539846 are labeled in pink and yellow, respectively. (B) 4C-seq analyses in MEC1 CLL cells indicate the formation of a loop domain between rs539846 and cis -regulatory elements. The 4C viewpoint (green box) lies adjacent to rs539846 (dotted line). A 10-kb masked region (gray box) is also marked. ENCODE ChIP-seq data from GM12878 cells show the correspondence between loop formation and CTCF and RELA transcription factor occupancy. Canonical transcripts and chromosome 15 position also are shown.
Article Snippet: Using 4C primer design software ( http://mnlab.uchicago.edu/4Cpd/ ), we identified a viewpoint adjacent to SNP rs539846.
Techniques: ChIP-sequencing, Labeling
Rao et al., 2014 ). The contact domain and CD19 + B cell super-enhancer (